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| Strain
Number: |
01BM2 |
| Common
Strain Name: |
K-rasLA1 |
| Strain
Nomenclature: |
B6.129S2-Krastm2Tyj |
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| Release
Category (Required for MTA form):
B3
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D
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| Strain
Information in Cancer Models Database |
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| Strain
Description: |
This strain carries a targeted latent 'hit-and-run' K-ras allele that can be activated by an in vivo spontaneous recombination event ('run'). One half of the in vivo recombination events result in a normal K-ras allele and one half in an activated allele (K-rasG12D). Mice carrying this mutation have a mean age of death/sacrifice of 300 days as a result of extensive tumor burden. The most frequent organ site is the lung. Varying grades of tumors are present from hyperplasia/dysplasia to carcinomas similar to human non-small cell lung cancer. Immunohistochemistry indicates tumors develop in alveolar type II cell lineage. Metastasis to thorasic lymph nodes, kidney and other visceral organs occurs with low frequency. Other organ sites include the thymus (thymic lymphoma) and skin (papillomas). A companion strain (K-rasLA2) carries an allele that recombines to the activated allele (K-rasG12D) 100% of the time. |
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| Mutation
Information |
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Mutation
Type: |
Targeted |
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Gene
Name: |
Kirsten rat sarcoma oncogene, 2 expressed
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Gene
Symbol: |
Kras
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Transgene
Name: |
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Transgene
Symbol: |
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
B6.129S2 |
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Approx.
Generation: |
21 |
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Organ
Site : |
Lung |
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Cryopreservation Mating Scheme: |
C57BL/6 females x heterozygous males |
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| Genotyping Information: |
Protocol 1: Allele: Kras
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| Donating
Investigator: |
Dr. Tyler Jacks |
| Key
Reference: |
Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA, Jacks. 2001. Somatic activation of the K-ras oncogene causes early onset lung cancer in mice.
Nature 410:1111-6.
PMID: 11323676
[PubMED
Abstract]
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