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Strain Number: 01BM3 
Common Strain Name: K-rasLA2 
Strain Nomenclature: B6.129S-Krastm3Tyj/Nci 
 
Release Category (Required for MTA form): B3 , D
Sample MTA for this strain
Animal Health Report in PDF Format  
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Strain Description: This strain carries a targeted latent 'hit-and-run' K-ras allele that can be activated by an in vivo spontaneous recombination event ('run'). The in vivo recombination event results in an activated allele (K-rasG12D). Mice carrying this mutation have a mean age of death/sacrifice of 200 days as a result of extensive tumor burden. The most frequent organ site is the lung. Varying grades of tumors are present from hyperplasia/dysplasia to carcinomas similar to human non-small cell lung cancer. Immunohistochemistry indicates tumors develop in alveolar type II cell lineage. Metastasis to thorasic lymph nodes, kidney and other visceral organs occurs with low frequency. Other organ sites include the thymus (thymic lymphoma) and skin (papillomas). A companion strain (K-rasLA1) carries an allele where one half of the in vivo recombination events result in a normal K-ras allele and one half in the K-rasG12D activated allele.
       
Mutation Information  
  Mutation Type: Targeted 
  Gene Name: Kirsten rat sarcoma oncogene, 2 expressed
  Gene Symbol: Kras
  Transgene Name:
  Transgene Symbol:
  Promoter Name:  
  Promoter Symbol:  
  Current Genetic Background: B6.129S2 
  Approx. Generation: 38 
  Organ Site : Lung 
       
Husbandry: Breeder pairs are supplied as a heterozygote (male or female) and a C57BL/6N mate.
Genotyping Information: Protocol 1: Allele: Kras  
       
Donating Investigator: Dr. Tyler  Jacks
Key Reference: Johnson L, Mercer K, Greenbaum D, Bronson RT, Crowley D, Tuveson DA, Jacks T. 2001. Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature.410:1111-6. PMID: 11323676 [PubMED Abstract]
     
       
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