| |
|
| |
|
| Strain
Number: |
01XA6 |
| Common
Strain Name: |
Tgfb1 Rag2 null |
| Strain
Nomenclature: |
STOCK Tgfb1tm1Doe Rag2tm1Fwa |
| |
|
|
| Release
Category (Required for MTA form):
B
|
|
| Animal Health Report in PDF Format |
|
| Strain
Information in Cancer Models Database |
|
|
|
|
| |
|
|
|
| Strain
Description: |
Tgfb1-/- Rag2-/- mice rapidly develop mucosal hyperplasia of the cecum and colon which progresses to adenoma and carcinoma. All mice are affected with multiple tumors by 5 months of age. Ceca and colon from Tgfb1+/+ Rag2-/- and Tgfb1+/- Rag2-/- exhibit hyperplasia, which in most cases does not develop further. There are no differences in cell proliferation, inflammation, or apoptosis in hyperplasic tissues from the three genotypes, indicating that the conversion to adenoma is a result of the inability to maintain epithelial tissue organization as opposed to loss of growth control, increased inflammatory activity or increased genetic instability. |
| |
|
|
|
| Mutation
Information |
|
| |
Mutation
Type: |
Targeted |
| |
Gene
Name: |
Recombination activating gene 2, Transforming growth factor, beta 1
|
| |
Gene
Symbol: |
Rag2, Tgfb1
|
| |
Transgene
Name: |
|
| |
Transgene
Symbol: |
|
| |
Promoter
Name: |
, |
| |
Promoter
Symbol: |
, |
| |
Current
Genetic Background: |
STOCK |
| |
Approx.
Generation: |
36 |
| |
Organ
Site : |
GI |
| |
|
|
|
|
Husbandry: |
Non-sibling breeders are provided as Tgfb1(+/-) Rag2(-/-) and Tgfb1(+/+) Rag2(-/-). Non-sibling matings are recommended to avoid inbreeding. |
| Genotyping Information: |
Protocol 1: Allele: Rag2
Protocol 1: Allele: Tgfb1
|
| |
|
|
|
| Donating
Investigator: |
Dr. Tom Doetschman |
| Key
Reference: |
Engle SJ, Hoying JB, Boivin GP, Ormsby I, Gartside PS, Doetschman T. 1999. Transforming growth factor beta1 suppresses nonmetastatic colon cancer at an early stage of tumorigenesis.
Cancer Res. 59:3379-86.
PMID: 10416598
[PubMED
Abstract]
|
| |
|
|
|
| |
|
|
|
|
