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| Strain
Number: |
01XAD |
| Common
Strain Name: |
Gadd45a null |
| Strain
Nomenclature: |
B6;129-Gadd45atm1Ajf |
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| Release
Category (Required for MTA form):
B
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| Animal Health Report in PDF Format |
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| Strain
Description: |
By homologous recombination a 3.65 kb segment of the Gadd45a gene, including upstream sequences and exons 1-3, were replaced with a PGK-neo cassette. Homozygous Gadd45a-deficient mice were born at Mendelian frequency and grew to adulthood normally. One year after 6-8 week-old mice had been subjected to ionizing radiation, the frquency of thymic lymphomas was 3x higher than in wild-type controls. Cell from Gadd45a-deficient mice show chromosomal instability. Approximately 1/2 of Gadd45a-deficient (homozygous) females in a mixed 129:B6 background did not breed successfully. Successful delivery in most in a pure B6 background.
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| Mutation
Information |
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Mutation
Type: |
Targeted |
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Gene
Name: |
Gadd45a
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Gene
Symbol: |
Gadd45a
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Transgene
Name: |
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Transgene
Symbol: |
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
B6;129 |
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Approx.
Generation: |
3 |
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Organ
Site : |
hematopoietic |
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Husbandry: |
Breeder pairs are supplied as a heterozygote (male or female) and wildtype C57BL/6 mate. Average litter size is six. 50% of homozygous females in mixed 129;B6 background died of delivery failure. This trait appears to not be present in the pure C57BL/6 background. |
| Genotyping Information: |
Protocol 1: Allele: Gadd45a
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| Donating
Investigator: |
Dr. Albert Fornace, Jr. |
| Key
Reference: |
Hollander MC; Sheikh MS; Bulavin DV; Lundgren K; Augeri-Henmueller L; Shehee R; Molinaro TA; Kim KE; Tolosa E; Ashwell JD; Rosenberg MP; Zhan Q; Fernandez-Salguero PM; Morgan WF; Deng CX; Fornace AJ Jr. Genomic instability in Gadd45a-deficient mice. Nat Genet 23, 176-184, 1999. PMID 10508513
[PubMED
Abstract]
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| Related
References: |
Hollander MC, Philburn RT, Patterson AD, Wyatt MA, Fornace AJ Jr. Genomic instability in Gadd45a-/- cells is coupled with S-phase checkpoint defects. Cell Cycle 4, 704-709, 2005. PMID 15846075. [PubMED
Abstract] |
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Hollander MC, Patterson AD, Salvador JM, Anver MR, Hunger SP, Fornace AJ. Gadd45a acts as a modifier locus for lymphoblastic lymphoma. Leukemia 19, 847-850, 2005. PMID 15744335.
[PubMED Abstract]
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Hollander MC, Kovalsky O, Salvador JM, Kim KE, Patterson AD, Haines DC, Fornace AJ Jr. Dimethylbenzanthracene carcinogenesis in Gadd45a-null mice is associated with decreased DNA repair and increased mutation frequency. Cancer Res 61, 2487-2491, 2001. PMID 11289119.
Hollander MC, Kovalsky O, Salvador JM, Kim KE, Patterson AD, Haines DC, Fornace AJ Jr. Dimethylbenzanthracene carcinogenesis in Gadd45a-null mice is associated with decreased DNA repair and increased mutation frequency. Cancer Res 61, 2487-2491, 2001. PMID 11289119.
[PubMED Abstract]
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| Sponsoring Institute: |
NIH Intramural Program |
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