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Strain Number: 01XBF 
Common Strain Name: TG6113 
Strain Nomenclature: B6;C3-TgTn(sb-T2/Onc2)6113Njen/Nci 
 
Release Category (Required for MTA form): C1
Sample MTA for this strain
   
       
Strain Description: The Sleeping Beauty mutagenic transposon [Tn(sb-T2/Onc2); T2/Onc2] was engineered to contain an engrailed-2 splice acceptor flanked by SB transposase binding sites that were optimized to increase transposition frequency. The transposon also contained splice acceptors and a bi-directional polyadenylation signal that can terminate transcription when integrated in either orientation into a gene. In addition, a murine stem cell virus (MSCV) long terminal repeat (LTR) and a splice donor were included to activate gene expression when integrated upstream of a gene. Two strains of transgenic T2/Onc2 mice were generated and screened for high transgene copy number and lack of transgene methylation. Strain 6113 [B6;C3- TgTn(sb-T2/Onc2)6113Njen] contained a transposon concatemer on chromosome 1.
       
Mutation Information  
  Mutation Type: Transgenic 
  Gene Name:
  Gene Symbol:
  Transgene Name: T2/Onc2 transposon (Sleeping Beauty)
  Transgene Symbol: T2/Onc2
  Promoter Name: MSCV LTR (viral) 
  Promoter Symbol: MSCV LTR 
  Current Genetic Background: B6;C3 
  Approx. Generation:
  Organ Site : Various 
       
Cryopreservation Mating Scheme: C57BL/6NCr females x heterozygous males  
Genotyping Information: Protocol 1: Allele: TgTn(sb-T2/Onc2)6113Njen  
       
Donating Investigator: Dr. Adam  Dupuy
Key Reference: Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321 [PubMED Abstract]
Related References: Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875. [PubMED Abstract]
     
Sponsoring Institute: National Cancer Institute  
       
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