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| Strain Number: | 01XBF | ||
| Common Strain Name: | TG6113 | ||
| Strain Nomenclature: | B6;C3-TgTn(sb-T2/Onc2)6113Njen | ||
| Release Category (Required for MTA form): C | |||
| Animal Health Report in PDF Format | |||
| Strain Description: | The Sleeping Beauty mutagenic transposon [Tn(sb-T2/Onc2); T2/Onc2] was engineered to contain an engrailed-2 splice acceptor flanked by SB transposase binding sites that were optimized to increase transposition frequency. The transposon also contained splice acceptors and a bi-directional polyadenylation signal that can terminate transcription when integrated in either orientation into a gene. In addition, a murine stem cell virus (MSCV) long terminal repeat (LTR) and a splice donor were included to activate gene expression when integrated upstream of a gene. Two strains of transgenic T2/Onc2 mice were generated and screened for high transgene copy number and lack of transgene methylation. Strain 6113 [B6;C3- TgTn(sb-T2/Onc2)6113Njen] contained a transposon concatemer on chromosome 1. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | T2/Onc2 transposon (Sleeping Beauty) | ||
| Transgene Symbol: | T2/Onc2 | ||
| Promoter Name: | MSCV LTR (viral) | ||
| Promoter Symbol: | MSCV LTR | ||
| Current Genetic Background: | C57BL/6 x C3H | ||
| Approx. Generation: | 2 | ||
| Organ Site : | various | ||
| Husbandry: | Breeder pairs are supplied as a hemizygote (male or female) and wildtype C57BL/6 mate. Average litter size is 8. | ||
| Genotyping Information: | Protocol 1: Allele: TgTn(sb-T2/Onc2)6113Njen | ||
| Donating Investigator: | Dr. Adam Dupuy | ||
| Key Reference: | Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321 [PubMED Abstract] | ||
| Related References: | Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875. [PubMED Abstract] | ||
| Sponsoring Institute: | National Cancer Institute | ||
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