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Strain Number: 01XBK 
Common Strain Name: RosaSB 
Strain Nomenclature: B6;129-Gt(ROSA)26Sortm1(sb11)Njen/Nci 
 
Release Category (Required for MTA form): C1
Sample MTA for this strain
   
       
Strain Description: To avoid epigenetic silencing often seen with transgenic insertions, the SB11 transposase was knocked into the ROSA26 locus (RosaSB). Relative to SB10, SB11 contains four amino acid substitutions that increase enzymatic activity. Expression of SB11 in RosaSB mice was equal in all tissues tested (brain, spleen, skin, lung). This strain is part of the improved Sleeping Beauty mutagenesis system developed by Nancy Jenkins' laboratory.
       
Mutation Information  
  Mutation Type: Targeted 
  Gene Name: ROSA26
  Gene Symbol: ROSA26
  Transgene Name:
  Transgene Symbol:
  Promoter Name:  
  Promoter Symbol:  
  Current Genetic Background: B6;129 
  Approx. Generation: 10 
  Organ Site : Various 
       
Cryopreservation Mating Scheme: C57BL/6NCr females x heterozygous males  
Genotyping Information: Protocol 1: Allele: Gt(ROSA)26Sor  
       
Donating Investigator: Dr. Adam  Dupuy
Key Reference: Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321 [PubMED Abstract]
Related References: Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875 [PubMED Abstract]
     
       
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