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| Strain
Number: |
01XBK |
| Common
Strain Name: |
RosaSB |
| Strain
Nomenclature: |
B6;129-Gt(ROSA)26Sortm1(sb11)Njen |
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| Release
Category (Required for MTA form):
C1
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| Strain
Description: |
To avoid epigenetic silencing often seen with transgenic insertions, the SB11 transposase was knocked into the ROSA26 locus (RosaSB). Relative to SB10, SB11 contains four amino acid substitutions that increase enzymatic activity. Expression of SB11 in RosaSB mice was equal in all tissues tested (brain, spleen, skin, lung). This strain is part of the improved Sleeping Beauty mutagenesis system developed by Nancy Jenkins' laboratory.
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| Mutation
Information |
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Mutation
Type: |
Targeted |
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Gene
Name: |
ROSA26
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Gene
Symbol: |
ROSA26
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Transgene
Name: |
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Transgene
Symbol: |
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
B6;129 |
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Approx.
Generation: |
10 |
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Organ
Site : |
Various |
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Cryopreservation Mating Scheme: |
C57BL/6NCr females x heterozygous males |
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| Genotyping Information: |
Protocol 1: Allele: Gt(ROSA)26Sor
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| Donating
Investigator: |
Dr. Adam Dupuy |
| Key
Reference: |
Dupuy AJ, Akagi K, Largaespada DA, Copeland NG, Jenkins NA. Mammalian mutagenesis using a highly mobile somatic Sleeping Beauty transposon system. Nature 436, 221-226, 2005. PMID:16015321
[PubMED
Abstract]
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| Related
References: |
Geurts AM; Collier LS; Geurts JL; Oseth LL; Bell ML; Mu D; Lucito R; Godbout SA; Green LE; Lowe SW; Hirsch BA; Leinwand LA; Largaespada DA. Gene mutations and genomic rearrangements in the mouse as a result of transposon mobilization from chromosomal concatemers. PLoS Genet 2, e156, 2006. PMID:17009875 [PubMED
Abstract] |
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