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| Strain
Number: |
01XBR |
| Common
Strain Name: |
Patched1(F1-2m) |
| Strain
Nomenclature: |
B6;129-Ptch1tm1Cklr |
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| Release
Category (Required for MTA form):
D
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| Animal Health Report in PDF Format |
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| Strain
Description: |
This Ptch1 conditional allele has been used to generate the pediatric malignant brain tumor Medulloblastoma (PMID 19213072) as well as the pediatric soft tissue sarcoma embryonal rhabdomyosarcoma (PMID 21316601). The allele is structural similar to the germline knockout allele of Ptch1 generated by Matthew P. Scott (PMID 9262482 ), which has been used in subsequent publications to generate medulloblastoma, rhabdomyosarcoma and basal cell carcinoma. |
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| Mutation
Information |
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Mutation
Type: |
Targeted |
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Gene
Name: |
patched 1
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Gene
Symbol: |
PTCH1
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Transgene
Name: |
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Transgene
Symbol: |
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
B6;129 |
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Approx.
Generation: |
1 |
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Organ
Site : |
Multiple |
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Husbandry: |
Breeder pairs are supplied as a heterozygous female x wildtype C57BL/6 male. According to information received from the donating investigator, Dr. Charles Keller, experimental animals are cancer-prone in the heterozygous (haploinsufficient) state. For experimentals, the Ptch1 conditional allele ALWAYS comes from the dam (Cre should come from sire). The strain being distributed is the Ptch1, not cre. Average litter size is 5-8. |
| Genotyping Information: |
Protocol 1: Allele: Ptch1
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| Donating
Investigator: |
Dr. Charles Keller |
| Key
Reference: |
Taniguchi, Min Jung Cho, Benjamin R. Arenkiel, Mark S. Hansen, Omar J. Rivera, Amanda T. McCleish, Stephen J. Qualman, Denis C. Guttridge, Matthew P. Scott, Mario R. Capecchi, Charles Keller. Bortezomib Reverses a Post-Translational Mechanism of Tumorigenesis for Patched1 Haploinsufficiency in Medulloblastoma, Pediatric Blood & Cancer, August 2009, (53)136-144 PMID 19213072
[PubMED
Abstract]
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| Related
References: |
Suresh I. Prajapati, Aoife Kilcoyne, Aislynn K. Samano, Dustin P. Green, Steven D. McCarthy, Barron A. Blackman, Michelle M. Brady, Lee Ann Zarzabal, Arun K.Tatiparthy, Timothy J. Sledz, Timothy Duong, Sachiko Ohshima-Hosoyama, Francis J. Giles, Joel E. Michalek, Brian P. Rubin, Charles Keller. Micro-CT Based Virtual Histology Evaluation of Preclinical Medulloblastoma. Molecular Imaging and Biology, 2011 Jun;13(3):493-9. PMID 20617390
[PubMED
Abstract] |
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Aislynn K.Samano, Sachiko Ohshima-Hosoyama, Thomas G. Whitney, Suresh I. Prajapati, Aoife Kilcoyne, Eri Taniguchi, William W. Morgan, Laura D. Nelon, Ai-Ling Lin, Osamu Togao, Inkyung Jung, Brian P. Rubin, Brent M. Nowak, Timothy Q. Duong, Charles Keller. Functional Evaluation of Therapeutic Response for a Mouse Model of Medulloblastoma. Transgenic Research, epub 2010 Jan 27 PMID 20107895
[PubMED Abstract]
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| Sponsoring Institute: |
1 K08 CA 90438-01 Keller (PI), NIH/NCI |
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