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| Strain Number: | 01XFA | ||
| Common Strain Name: | MH19 | ||
| Strain Nomenclature: | FVB/N-Tg(MT-HGFSF)19Lmb | ||
| Release Category (Required for MTA form): C1 , D | |||
| Strain Description: | MH19 is a transgenic line that overexpresses mouse Scatter factor/hepatocyte growth factor under control of the mouse metallothionein I promoter. Expression can be detected in virtually all adult tissues, at a level between 3- and 50-fold higher than the endogenous gene, Hgf (Takayama et al., 1996). Over half of the female transgenic mice die by 6 months of age of intestinal obstruction or renal failure. Hind limb paralysis due to ectopic development of striated muscle in the central nervous system was observed in 5% of the animals at 4.5-6.5 weeks of age (Takayama et al., 1996). MH19 mice developed multiple tumors at 2.5-20 months of age (Takayama et al., 1997). Lesions were of epithelial and mesenchymal origin. They included skin fibrosarcomas, hepatocellular adenomas, hemangiosarcomas, rhabdomyosarcomas, mammary tumors (Takayama et al., 1997), and malignant melanoma (Otsuka et al., 1998). MH19 mice tend to develop a rectal prolapse, which occasionally can be severe. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | Scatter factor/hepatocyte growth factor | ||
| Transgene Symbol: | SCATTER | ||
| Promoter Name: | metallothionein (mouse) | ||
| Promoter Symbol: | mMT-1 | ||
| Current Genetic Background: | FVB/N | ||
| Approx. Generation: | 8 | ||
| Organ Site : | |||
| Cryopreservation Mating Scheme: | FVB/NCr females x heterozygous males | ||
| Genotyping Information: | Protocol 1: Allele: Tg(MT-HGFSF)19Lmb | ||
| Donating Investigator: | Dr. Glenn Merlino | ||
| Key Reference: | Takayama H, LaRochelle WJ, Anver M, Bockman DE, Merlino G. Scatter factor/hepatocyte growth factor as a regulator of skeletal muscle and neural crest development. Proc Natl Acad Sci USA 93, 5866-5871, 1996. PMID:8650184 [PubMED Abstract] | ||
| Related References: | Takayama H, LaRochelle WJ, Sharp R, Otsuka T, Kriebel P, Anver M, Aaronson SA, Merlino G. Diverse tumorigenesis associated with aberrant development in mice overexpressing hepatocyte growth factor/scatter factor. Proc Natl Acad Sci USA 94, 701-706, 1997. PMID:9012848 [PubMED Abstract] | ||
| Otsuka T, Takayama H, Sharp R, Celli G, LaRochelle WJ, Bottaro DP, Ellmore N, Vieira W, Owens JW, Anver M, Merlino G. C-Met autocrine activation induces development of malignant melanoma and acquisition of the metastatic phenotype. Cancer Res 58, 5157-5167. PMID: 9823327 [PubMED Abstract] | |||
| Sponsoring Institute: | NCI/CCR Research Funds | ||
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