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Strain Number: 01XGC 
Common Strain Name: Rosa LSL SBase/TG12775 
Strain Nomenclature: STOCK Gt(ROSA)26Sortm2(sb11)Njen TgTn(sb-T2/Onc3)12775Njen/Nci 
Release Category (Required for MTA form): C1
Sample MTA for this strain
Animal Health Report in PDF Format  
Strain Description: This strain is double homozygous for both the TgTn(sb-T2/Onc3)12775Njen (TG12775) Sleeping Beauty mutagenic transgene and a new cre-inducible SBase knock-in allele at ROSA26. This double homozygous strain has a genotype designation of lsl-SBase/lsl-SBase; SB/SB, where lsl-SBase refers to the cre-inducible SBase allele at ROSA26 and SB refers to the TG12775 transgene. Homozygosity for the cre-inducible SBase allele at ROSA26 was determined by PCR genotyping and homozygosity for the TG12775 transgene was determined by a combination of PCR and FISH genotyping; both alleles were confirmed homozygous by test crossing to wild-type mice. A major advantage of the bi-partite Sleeping Beauty mutagenesis system is the ability to selectively control transposase (SBase) expression both spatially and temporally. To create the cre-inducible SBase knock-in allele ROSA26, the same approach was taken as before (see MMHCC 01XBG), except that a floxed-STOP (loxP-STOP-loxP) cassette was inserted upstream of the SBase (SB11) gene, thereby preventing SBase expression. Tissue-specific SB expression may be controlled easily by crossing with various cre-expressing mice. Importantly, after deletion of the floxed-STOP cassestte by cre-loxP recombination, SB functions in the same manner as the constitutive SBase allele (NCI Mouse Repository Strain 01XBG). The Sleeping Beauty mutagenic transposon [Tn(sb-T2/Onc3); T2/Onc3] was engineered to contain an engrailed-2 splice acceptor flanked by SB transposase binding sites that were optimized to increase transposition frequency. The transposon also contained splice acceptors and a bi-directional polyadenylation signal that can terminate transcription when integrated in either orientation into a gene. A CMV enhancer/chicken ß-actin promoter that drives oncogene expression and a splice donor were included to activate gene expression when integrated upstream of a gene. Two strains of transgenic T2/Onc3 mice were generated: strain TG12775 [B6;C3- TgTn(sb-T2/Onc3)12775Njen] contains a transposon concatemer on chromosome 12. This double homozygous strain is part of the next generation Sleeping Beauty mutagenesis system developed in Nancy Jenkins' laboratory.
Mutation Information  
  Mutation Type: Targeted 
  Gene Name: ROSA26, sleeping beauty transposon (mouse)
  Gene Symbol: ROSA26, sb
  Transgene Name:
  Transgene Symbol:
  Promoter Name: ,  
  Promoter Symbol: ,  
  Current Genetic Background: STOCK 
  Approx. Generation: homo 
  Organ Site : Various 
Husbandry: Breeder pairs are supplied as homozygotes; average litter size is 6. Strain is maintained as homozygous and cannot be readily genotyped by PCR. It can, however, be genotyped by FISH analysis.
Genotyping Information: There are currently no protocols available for this strain.  
Donating Investigator: Dr. Nancy  Jenkins
Key Reference: Dupuy AJ, Rogers LM, Kim J, Nannapaneni K, Starr TK, Liu P, Largaespada DA, Scheetz TE, Jenkins NA, Copeland NG. A modified sleeping beauty transposon system that can be used to model a wide variety of human cancers in mice. Cancer Res. 2009 Oct 15;69(20):8150-6. Epub 2009 Oct 6. PMID: 19808965 [PubMED Abstract]
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