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Strain Number: 01XJ6 
Common Strain Name: LSL K-ras G12D 
Strain Nomenclature: B6.129-Krastm4Tyj/Nci 
 
Release Category (Required for MTA form): B3 , D
Sample MTA for this strain
Animal Health Report in PDF Format  
   
       
Strain Description: These mice carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.
Intranasal infection with an adenovirus encoding Cre resulted in a very high frequency of lung tumors. Homozygotes die in utero.
       
Mutation Information  
  Mutation Type: Targeted 
  Gene Name: Kirsten rat sarcoma oncogene, 2 expressed
  Gene Symbol: Kras
  Transgene Name:
  Transgene Symbol:
  Promoter Name:  
  Promoter Symbol:  
  Current Genetic Background: B6.129 
  Approx. Generation: 18 
  Organ Site : Lung 
       
Husbandry: Breeder pairs are supplied as a heterozygote (male or female) x wildtype C57BL/6 mate. This Kras allele is non-functional in its germline configuration, therefore the mice are maintained by backcrossing heterozygous animals to C57BL/6. Average litter size is 9.
Genotyping Information: Protocol 1: Allele: Kras Protocol 2: Allele: Kras  
       
Donating Investigator: Dr. Tyler  Jacks
Key Reference: Jackson EL, Willis N, Mercer K,Bronson RT, Crowley D, Montoya R, Jacks T, and Tuveson DA. 2001. Analysis of lung tumor initiation and progression using conditional expression of K-ras. Genes and Development 15: 3243-3248 PMID: 11751630 [PubMED Abstract]
Related References: Johnson L, Mercer K, Greenbaum D, Bronsont RT, Crowley D, Tuveson DA, and Jacks T. 2001 Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410:1111-1116 PMID: 11323676 [PubMED Abstract]
Tuveson DA, Shaw AT, Willis NA, Silver DP, Jackson EL, Chang S, Mercer KL, Grochow R, Hock H, Crowley D, Hingorani SR, Zaks T, King C, Jacobetz MA, Wang L, Bronson RT, Orkin SH, DePinho RA, Jacks T. 2004. Endogenous oncogenic K-ras(G12D) stimulates proliferation and widespread neoplastic and developmental defects. Cancer Cell 5, 375-378. PMID:15093544 [PubMED Abstract]
     
       
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