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| Strain
Number: |
01XJ6 |
| Common
Strain Name: |
LSL K-ras G12D |
| Strain
Nomenclature: |
B6.129-Krastm4Tyj |
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| Release
Category (Required for MTA form):
B3
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D
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| Animal Health Report in PDF Format |
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| Strain
Description: |
These mice carry a latent point-mutant allele of Kras2 (K-rasG12D). Cre-mediated recombination leads to deletion of a transcriptional termination sequence (Lox-Stop-Lox) and expression of the oncogenic protein.
Intranasal infection with an adenovirus encoding Cre resulted in a very high frequency of lung tumors. Homozygotes die in utero. |
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| Mutation
Information |
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Mutation
Type: |
Targeted |
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Gene
Name: |
Kirsten rat sarcoma oncogene, 2 expressed
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Gene
Symbol: |
Kras
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Transgene
Name: |
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Transgene
Symbol: |
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Promoter
Name: |
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Promoter
Symbol: |
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Current
Genetic Background: |
B6.129 |
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Approx.
Generation: |
15 |
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Organ
Site : |
Lung |
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Husbandry: |
Breeder pairs are supplied as a heterozygote (male or female) x wildtype C57BL/6 mate. This Kras allele is non-functional in its germline configuration, therefore the mice are maintained by backcrossing heterozygous animals to C57BL/6. Average litter size is 9. |
| Genotyping Information: |
Protocol 1: Allele: Kras
Protocol 2: Allele: Kras
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| Donating
Investigator: |
Dr. Tyler Jacks |
| Key
Reference: |
Jackson EL, Willis N, Mercer K,Bronson RT, Crowley D, Montoya R, Jacks T, and Tuveson DA. 2001. Analysis of lung tumor initiation and progression using conditional expression of K-ras. Genes and Development 15: 3243-3248
PMID: 11751630
[PubMED
Abstract]
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| Related
References: |
Johnson L, Mercer K, Greenbaum D, Bronsont RT, Crowley D, Tuveson DA, and Jacks T. 2001 Somatic activation of the K-ras oncogene causes early onset lung cancer in mice. Nature 410:1111-1116
PMID: 11323676 [PubMED
Abstract] |
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Tuveson DA, Shaw AT, Willis NA, Silver DP, Jackson EL, Chang S, Mercer KL, Grochow R, Hock H, Crowley D, Hingorani SR, Zaks T, King C, Jacobetz MA, Wang L, Bronson RT, Orkin SH, DePinho RA, Jacks T. 2004. Endogenous oncogenic K-ras(G12D) stimulates proliferation and widespread neoplastic and developmental defects. Cancer Cell 5, 375-378. PMID:15093544
[PubMED Abstract]
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