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| Strain Number: | 01XT4 | ||
| Common Strain Name: | iDCT-GFP | ||
| Strain Nomenclature: | FVB.Cg-Tg(Dct-rtTA2S*M2)#Mrln Tg(tetO-HIST1H2BJ/GFP)47Efu/Nci | ||
| Release Category (Required for MTA form): B1 | |||
| Animal Health Report in PDF Format | |||
| Strain Description: | The mice display normal wildtype phenotype. Administration of doxycycline, by diet or injection, induces expression of GFP in the melanocytes in the hair follicles of the skin. In embryonic stages, GFP+ cells arise at ~E10.5 and gradually spread through the neural crest, retinal pigment epithelium, and telencephalon in the brain, consistent with the known expression pattern of the Dct gene. Due to the fusion with histone H2B, GFP fluorescent signal in the cells is distinctly nuclear. | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | Mouse histone H2B fused with green fluorescent protein from jellyfish, Tetracycline reverse transcriptional activator | ||
| Transgene Symbol: | H2B-GFP, rtTA | ||
| Promoter Name: | , Dopachrome tautomerase (Dct) | ||
| Promoter Symbol: | , Dct | ||
| Current Genetic Background: | FVB.Cg | ||
| Approx. Generation: | Homo | ||
| Organ Site : | |||
| Husbandry: | Homozygote breeder pairs are provided. The Donating Investigator recommends using 1-2 females per male. | ||
| Genotyping Information: | Protocol 1: Allele: Tg(Dct-rtTA2S*M2)#Mrln Protocol 1: Allele: Tg(tetO-HIST1H2BJ/GFP)46Efu/Nc | ||
| Donating Investigator: | Dr. Glenn Merlino | ||
| Key Reference: | Zaidi, M.R., et al., Interferon-gamma links ultraviolet radiation to melanomagenesis in mice. Nature, 2011. 469(7331): p. 548-53.PMID: 21248750 [PubMED Abstract] | ||
| Related References: | Zaidi, M.R., et al., A genetically engineered mouse model with inducible GFP expression in melanocytes. Pigment Cell & Melanoma Research, 2011. 24 (2):p. 393. PMID: 21392368 [PubMED Abstract] | ||
| Sponsoring Institute: | Intramural Research Program, National Cancer Institute. | ||
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