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Strain Number: 01XT5 
Common Strain Name: Rosa LSL SBase/TG6070 
Strain Nomenclature: STOCK Gt(ROSA)26Sortm2(sb11)Njen TgTn(sb-T2/Onc2)6070Njen/Nci 
 
Release Category (Required for MTA form): C1
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Strain Description: This strain is double homozygous for both the TgTn(sb-T2/Onc2)6070Njen (TG6070) Sleeping Beauty mutagenic transgene and a new cre-inducible SBase knock-in allele at ROSA26. This double homozygous strain has a genotype designation of lsl-SBase/lsl-SBase; SB/SB, where lsl-SBase refers to the cre-inducible SBase allele at ROSA26 and SB refers to the TG6070 transgene. Homozygosity for the cre-inducible SBase allele at ROSA26 was determined by PCR genotyping and homozygosity for the TG6070 transgene was determined by a combination of PCR and FISH genotyping; both alleles were confirmed homozygous by test crossing to wild-type mice. A major advantage of the bi-partite Sleeping Beauty mutagenesis system is the ability to selectively control transposase (SBase) expression both spatially and temporally. To create the cre-inducible SBase knock-in allele ROSA26, the same approach was taken as before (see NCI Mouse Repository Strain 01XBG), except that a floxed-STOP (loxP-STOP-loxP) cassette was inserted upstream of the SBase (SB11) gene, thereby preventing SBase expression. Tissue-specific SB expression may be controlled easily by crossing with various cre-expressing mice. Importantly, after deletion of the floxed-STOP cassestte by cre-loxP recombination, SB functions in the same manner as the constitutive SBase allele (NCI Mouse Repository Strain 01XBG). The Sleeping Beauty mutagenic transposon [TgTn(sb-T2/Onc2)6070Njen] is the same allele as described in NCI Mouse Repository Strain Number 01XBG. This double homozygous strain is part of the next generation Sleeping Beauty mutagenesis system developed in Nancy Jenkins' laboratory.
       
Mutation Information  
  Mutation Type: Targeted 
  Gene Name: ROSA26, sleeping beauty transposon (mouse)
  Gene Symbol: ROSA26, sb
  Transgene Name:
  Transgene Symbol:
  Promoter Name: ,  
  Promoter Symbol: ,  
  Current Genetic Background: STOCK 
  Approx. Generation: homo 
  Organ Site : Various 
       
Husbandry: Homozygous breeder pairs are provided. Average litter size is 6. Strain is maintained as homozygous and cannot be readily genotyped by PCR. It can, however, be genotyped by FISH analysis.
Genotyping Information: There are currently no protocols available for this strain.  
       
Donating Investigator: Dr. Nancy  Jenkins
Key Reference: A transposon-based genetic screen in mice identifies genes altered in colorectal cancer. Starr TK, Allaei R, Silverstein KA, Staggs RA, Sarver AL, Bergemann TL, Gupta M, O'Sullivan MG, Matise I, Dupuy AJ, Collier LS, Powers S, Oberg AL, Asmann YW, Thibodeau SN, Tessarollo L, Copeland NG, Jenkins NA, Cormier RT, Largaespada DA. Science. 2009 Mar 27;323(5922):1747-50. Epub 2009 Feb 26. PMID: 19251594 [PubMED Abstract]
     
       
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