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| Strain Code: | 01XM8 | ||
| Common Strain Name: | K5/tTA | ||
| Strain Nomenclature: | FVB/N-Tg(KRT5-tTA)1216Glk | ||
| Strain Description: | The doxycycline-dependent transcriptional repressor, tTA, was placed under control of the bovine keratin 5 (K5, KRT5) promoter. When tested on a tetO-LacZ transgenic background, doxycycline suppressed expression of beta-galactosidase in a tissue-specific manner. Reduced beta-galactosidase activity was detected in skin (basal layer of the epidermis and hair follicles), tongue, forestomach, and trachea. No reduction was seen in tissues that do not express endogenous keratin 5 (liver, spleen, muscle, heart, intestine). | ||
| Mutation Information | |||
| Mutation Type: | Transgenic | ||
| Gene Name: | |||
| Gene Symbol: | |||
| Transgene Name: | doxycylin-dependent transcriptional repressor, tTA | ||
| Transgene Symbol: | KRT5 | ||
| Promoter Name: | keratin 5 (bovine) | ||
| Promoter Symbol: | K5 | ||
| Current Genetic Background: | FVB/N | ||
| Approx. Generation: | 3 | ||
| Organ Site : | Multiple | ||
| Donating Investigator: | Adam Glick | ||
| Reference: | Diamond I, Owolabi T, Marco M, Lam C, Glick A. Conditional gene expression in the epidermis of transgenic mice using the tetracyclin-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol 115, 788-794, 2000. PMID: 11069615 | ||
| PubMED Abstract | |||
| Sponsoring Institute: | National Cancer Institute | ||
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