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Newly Accepted Strain Details

Strain Code: 01XM8 
Common Strain Name: K5/tTA 
Strain Nomenclature: FVB/N-Tg(KRT5-tTA)1216Glk/Nci 
       
       
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Strain Description: The doxycycline-dependent transcriptional repressor, tTA, was placed under control of the bovine keratin 5 (K5, KRT5) promoter. When tested on a tetO-LacZ transgenic background, doxycycline suppressed expression of beta-galactosidase in a tissue-specific manner. Reduced beta-galactosidase activity was detected in skin (basal layer of the epidermis and hair follicles), tongue, forestomach, and trachea. No reduction was seen in tissues that do not express endogenous keratin 5 (liver, spleen, muscle, heart, intestine).
       
Mutation Information  
  Mutation Type: Transgenic 
  Gene Name:  
  Gene Symbol:  
  Transgene Name: doxycylin-dependent transcriptional repressor, tTA  
  Transgene Symbol: KRT5  
  Promoter Name: keratin 5 (bovine) 
  Promoter Symbol: K5 
  Current Genetic Background: FVB/N 
  Approx. Generation:
  Organ Site : Multiple 
       
Donating Investigator:  Adam  Glick
Reference: Diamond I, Owolabi T, Marco M, Lam C, Glick A. Conditional gene expression in the epidermis of transgenic mice using the tetracyclin-regulated transactivators tTA and rTA linked to the keratin 5 promoter. J Invest Dermatol 115, 788-794, 2000. PMID: 11069615
    PubMED Abstract  
       
Sponsoring Institute: National Cancer Institute  
       
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